Journal: iScience

Article Title: Glycolysis maintains effector function of lung Th17 TRM cells in precision cut lung slices

doi: 10.1016/j.isci.2025.114051

Figure Lengend Snippet: Long-lived lung CD4 + Th17 cells are enriched in glycolysis over time (A) Cell number of OVA + cells after tetramer pulldown ( n = 2). (B) Gene set enrichment analysis between day 30 and day 90 OVA + CD4 + T cells. (C) GSEA result comparing day 30 and day 90 OVA + CD4 + T cells. (D) GSEA result comparing day 30 and day 90 OVA + Il17a + CD4 + T cells. (E) GSEA result comparing human lung CD4 + TRM cells and naive CD4 + T cells from lymph nodes. GEO: GSE137967 . (F) Dot plot showing scaled expression of key glycolytic genes ( Hk2, Pfkp, Aldoa, Gapdh, Pgk1, Eno1, Pkm, Ldha, and Galm ) in day 30 vs. day 90 OVA + IL-17A + CD4 + T cells. (G) Heatmap of key glycolytic genes ( HK2, PFKP, ALDOA, GAPDH, PGK1, ENO1, PKM, LDHA, and GALM ) in human lung CD4 + TRM cells and naive CD4 + T cells from lymph nodes.

Article Snippet: Rabbit anti-IL17A antibody , nsj Bioreagents , Cat# RQ4021.

Techniques: Expressing

Journal: iScience

Article Title: Glycolysis maintains effector function of lung Th17 TRM cells in precision cut lung slices

doi: 10.1016/j.isci.2025.114051

Figure Lengend Snippet: Metabolic features of TRM cells are maintained in PCLS (A) In vivo labeling of glucose uptake using 2-NBDG on lung CD4 + CD69 + TRM cells. Mice were immunized with OmpX+LTA1 as described before. On day 30 after prime immunization, mice were given 500 nmol 2-NBDG by intratracheal oropharyngeal aspiration. Mice were sacrificed 30 min later. Lungs were harvested for digestion and flow cytometry. (B) Cartoon depicting immunization schedule with mouse PCLS generation. (C) CD3 + CD69 + IL-17 + TRM cells in PCLS of LTA1/OmpX-immunized C57BL/6 mice, compared with naive mice, were visualized with multicolor immunofluorescence (scale bars: 100 μm). (D–F) RNA was extracted from PCLS. Il17a expression was measured by real-time RT-qPCR. ( n = 4–8 PCLS per group). Data represent two independent experiments. Data are presented as means ± SEM. Significant differences were calculated with Mann-Whitney test or one-way ANOVA. ∗, p ≤ 0.05, ∗∗, p ≤ 0.01, ∗∗∗, p ≤ 0.001, and ∗∗∗∗, p ≤ 0.0001.

Article Snippet: Rabbit anti-IL17A antibody , nsj Bioreagents , Cat# RQ4021.

Techniques: In Vivo, Labeling, Flow Cytometry, Immunofluorescence, Expressing, Quantitative RT-PCR, MANN-WHITNEY